6th Meeting of the Panel on Quarantine Nematodes
Firenze, IT, 2009-03-31/04-02
The Panel on Quarantine Nematodes met at the Research Center for Agrobiology and Pedology of the Agricultural Research Council of Emilia-Romagna at the kind invitation of Ms Curto. The Panel continued its work on the preparation of diagnostic protocols. To date, 8 diagnostic protocols for nematodes have been published.
The comments received from country consultation on the Diagnostic protocol for Xiphinema americanum (sensus lato) and Hirschmanniella spp. were reviewed and the protocols will be presented for final adoption in 2009.
Revisions of the protocols for Bursaphelenchus xylophilus, Globodera rostochiensis & pallida and Nacobbus aberrans were prepared and the Panel considered them ready to be sent for country consultation.
- The protocol for B. xylophilus has been revised to include new molecular methods. Simplified morphological keys have also been added to the protocols. This revision was considered to be a priority.
- The protocol for Globodera rostochiensis & G. pallida has been revised to include real-time PCR tests and an appendix on testing cyst viability.
- The protocol for Nacobbus aberrans has also been revised to include molecular tests and some changes regarding morphological identification.
The Panel agreed that these protocols should be presented for final approval in 2009 if the EPPO Secretariat is able to address comments made during country consultation together with the authors.
The Panel reviewed a pictorial glossary which aims at explaining the morphological terms used in the protocols for nematodes (this document which will not be a standard should be published on the EPPO website alongside protocols).
The Panel finalized a standard on soil tests for virus-vector nematodes in the framework of EPPO standards on the production of healthy plants for planting (PM 4). In particular, it prepared a section on sampling and extraction for nematodes. The Panel considered that the document was ready to be sent for country consultation.
The Panel was informed of the latest developments concerning the EPPO programme on quality assurance and accreditation and in particular on the new EPPO standard on Specific requirements for laboratories preparing for accreditation for plant pest diagnosis activities. The process of validation of tests was presented, as well as tables giving indications on the number of samples, replicates and repetitions suitable for validation.
An overview about the situation of Meloidogyne species such as Meloidogyne ulmi, M. minor and M. enterolobii in Europe was given. The Panel noted that a PRA had been conducted in the Netherlands on M. enterolobii and that an EPPO PRA will be prepared in May 2009. A joint Dutch/British PRA on M. minor concluded that potato, rotation pasture and managed turf were at risk, and that the species complex was not fully characterized and could be easily confused with other species such as M. chitwoodi. As surveys are required to identify the distribution in the European countries, the addition of M. minor to the Alert List was suggested.
The next meeting of the Panel on Quarantine Nematodes is scheduled for 2010-12-07/09 in Zürich (CH), at the kind invitation of Mr Kiewnick.
Courtesy: Sue Hockland (FERA, GB)
PARTICIPANTS
Anthoine Géraldine (Ms)
Laboratoire National de la Protection des Végétaux- Unité de Nématologie, Domaine de la Motte au Vicomte - BP 35327, 35653 Le Rheu Cedex (FR) - +33-223485208 - +33-223485628 - geraldine.anthoine@rennes.inra.fr
Curto Giovanna (Ms)
Plant Protection Service - Servizio Fitosanitario Regione Emilia-Romagna, Via di Saliceto, n.81, 40128 Bologna (IT) - +39-0514159218 - +39-0514159277 - gcurto@regione.emilia-romagna.it
Darnadyova Kristina (Ms)
UKSUP, Section of Diagnostics, Central Control and Testing Institute of Agriculture, Letna 3, 04139 Kosice (SK) - +421-557901253 - +421-557901201 - kristina.darnadyova@zoznam.sk
Gaar Vladimir (Mr)
State Phytosanitary Administration, Diagnostic Laboratory, Drnovska 73/507, 161 06 Praha 6 (CZ) - +420-233022343 - +420-724121293 - +420-233022343 - vladimir.gaar@srs.cz
Hallmann Johannes (Mr)
Julius Kühn Institut (JKI), Federal Research Centre for Cultivated Plants, Institut für Epidemiologie und Pathogendiagnostik, Toppheideweg 88, 48161 Münster (DE) - +49-2518710625 - +49-2518710633 - johannes.hallmann@jki.bund.de
Hockland Sue Hazel (Ms)
Food and Environment Research Agency, room 02FA02, Sand Hutton, YO41 1LZ York (GB) - +44-1904462214 - +44-1904462250 - s.hockland@csl.gov.uk
Kahrer Andreas (Mr)
Austrian Agency for Health and Food Safety (AGES), Institute for Plant Health, Spargelfeldstrasse 191, A-1226 Vienna (AT) - +43-5055533321 - +43-5055533303 - andreas.kahrer@ages.at
Karnkowski Witold (Mr)
Main Inspectorate of Plant Protection and Seed Service, Centralner Laboratorium, ul.Zwirki i Wigugy 73, 87100 Torun (PL) - +48-566235698 - +48-566528228 - w.karnkowski@piorin.gov.pl
Karssen Gerrit (Mr)
Plant Protection Service, P.O. Box 9102, 6700 HC Wageningen (NL) - +31-317496846 - +31-317421701 - g.karssen@minlnv.nl
Kiewnick Sebastian (Mr)
Agroscope Changins-Wädenswil ACW, Research Station ACW, Plant Protection Ecotoxicology and Soil Zoology, Schloss, Laboratory 4, P.O. Box, 8820 Wädenswil (CH) - +41-447836336 - +41-447836434 - sebastian.kiewnick@acw.admin.ch
Magnusson Christer (Mr)
Norwegian Institute for Agricultural and Environmental Research, Hogskoleveien 7, 1432 Aas (NO) - +47-95205304 - +47-649446110 - christer.magnusson@bioforsk.no
Manduric Sanja (Ms)
SLU, Departement of Crop Science, Box 44, 23053 Alnarp (SE) - +46-40415261 - +46-40462166 - sanja.manduric@vv.slu.se
Pylypenko Liliya (Ms)
Plant Quarantine Department of Institute of Plant Protection Ukrainian Academy of Agrarian Sciences, 33 Vasilkovskaya Str., 03022 Kiev - 22 (UA) - +38044 2571360; 2571124 - +38044 2572185 - pylypenkol@mail.ru
Sirca Sasa (Mr)
Agricultural Institute of Slovenia, Hacqetova 17, 1000 Ljubljana (SI) - +386-12805255 - +396-12805255 - sasa.sirca@kis.si
Smaha Djamel (Mr)
Laboratoire de Nématologie, Institut National de Protection des Végétaux (INPV), Avenue Hacen Badi, B.P. 80, 16200 El Harrach Alger (DZ) - nemaalg01@yahoo.fr
Viaene Nicole (Ms)
ILVO, Plant-Crop Protection, Nematology, Burg. Van Gansberghelaan 96, 9820 Merelbeke (BE) - +32-92722425 - +32-92722429 - nicole.viaene@ilvo.vlaanderen.be
EPPO
Petter Françoise (Ms)
Sunley Robert (Mr)
OEPP/EPPO, 1 rue le Nôtre, 75016 PARIS (FR) - +01-45207794 - +0142248943 - hq@eppo.fr
Observers
Carletti Beatrice (Ms)
Research Centre for Agrobiology and Pedology of the Agricultural Research Council (CRA-ABP), Via Lanciola 12/A, Cascine del Riccio, 50125 Florence (IT) - +39 0552492250 - beatrice.carletti@isza.it
Damme Nicole (Ms)
ILVO, Plant-Crop Protection, Nematology, Burg. van Gansberghelaan 96 bus 2, B-9820 Merelbeke (BE) - +32-92722444 - +32-92722429 - nicole.damme@ilvo.vlaanderen.be